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KMID : 0604520080340030183
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Journal of the Society of Cosmetic Scientists of Korea
2008 Volume.34 No. 3 p.183 ~ p.188
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Reliability of In Vitro Assay for Initial Depigmenting Agent Screening
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H Dung-Nguyen
Lee Hyang-Bok
Shin Jeong-Hyun
Kim Eun-Ki
Min Eun-Yen
La Lyun-Hwa
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Abstract
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Initial screening assay for depigmenting agents includes in vitro mushroom tyrosinase assay and antioxidant assay. Based on this screening result, melanin synthesis in melanocyte, in screened samples, is further measured. Measuring cellular melanin needs time, human resource, and skills. Therefore initial screening method should be reliable. We examined, 34 Chinese herbs, correlated the screening assay methods with cellular melanin. No reliable relationship was observed between factors, indicating the limitation in the use of these assays, probably due to the complexicity of melanogenesis.
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KEYWORD
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depigmenting agents, screening, mushroom tyrosinase, antioxidant, melanin
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